Paracrine effects of mir-210-3p on angiogenesis in hypoxia-treated c-kit-positive cardiac cells.

ABSTRACT: Objective: Treatment with c-kit-positive cardiac cells (CPCs) has been shown to improve the prognosis of ischemic heart disease. MicroRNAs (miRNAs) confer protection by enhancing the cardiac repair process, but their specific functional mechanisms remain unclear. This study aimed to screen for differentially expressed miRNAs in CPCs under hypoxia and explore their effects on the function of CPCs.Methods: We harvested CPCs from C57 adult mice and later performed a high-throughput miRNA sequencing for differential expression profiling analysis. Subsequently, we intervened with the differentially expressed gene miR-210-3p in CPCs and detected changes in the secretion of angiogenesis-related factors through a protein-chip analysis. Finally, we applied CPC supernatants of differen

SUBMITTER: Shen L 

PROVIDER: S-EPMC10364570 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature