Cerebellar axonopathy in Shivers horses identified by spatial transcriptomic and proteomic analyses.

ABSTRACT:

Background

Shivers in horses is characterized by abnormal hindlimb movement when walking backward and is proposed to be caused by a Purkinje cell (PC) axonopathy based on histopathology.

Objectives

Define region-specific differences in gene expression within the lateral cerebellar hemisphere and compare cerebellar protein expression between Shivers horses and controls.

Animals

Case-control study of 5 Shivers and 4 control geldings ≥16.2 hands in height.

Methods

Using spatial transcriptomics, gene expression was compared between Shivers and control horses in PC soma and lateral cerebellar hemisphere white matter, consisting primarily of axons. Tandem-mass-tag (TMT-11) proteomic analysis was performed on lateral cerebellar hemisphere homogenates.

Results

D

SUBMITTER: Valberg SJ 

PROVIDER: S-EPMC10365050 | biostudies-literature | 2023 Jul-Aug

REPOSITORIES: biostudies-literature