High-throughput spatiotemporal monitoring of single-cell secretions via plasmonic microwell arrays.

ABSTRACT: Methods for the analysis of cell secretions at the single-cell level only provide semiquantitative endpoint readouts. Here we describe a microwell array for the real-time spatiotemporal monitoring of extracellular secretions from hundreds of single cells in parallel. The microwell array incorporates a gold substrate with arrays of nanometric holes functionalized with receptors for a specific analyte, and is illuminated with light spectrally overlapping with the device's spectrum of extraordinary optical transmission. Spectral shifts in surface plasmon resonance resulting from analyte-receptor bindings around a secreting cell are recorded by a camera as variations in the intensity of the transmitted light while machine-learning-assisted cell tracking eliminates the influence of cell movemen

SUBMITTER: Ansaryan S 

PROVIDER: S-EPMC10365996 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature