Characterization of Microtubule Lattice Heterogeneity by Segmented Subtomogram Averaging.

ABSTRACT: Microtubule structure is commonly investigated using single-particle analysis (SPA) or subtomogram averaging (STA), whose main objectives are to gather high-resolution information on the αβ-tubulin heterodimer and on its interactions with neighboring molecules within the microtubule lattice. The maps derived from SPA approaches usually delineate a continuous organization of the αβ-tubulin heterodimer that alternate regularly head-to-tail along protofilaments, and that share homotypic lateral interactions between monomers (α-α, β-β), except at one unique region called the seam, made of heterotypic ones (α-β, β-α). However, this textbook description of the microtubule lattice has been challenged over the years by several studies that revealed the presence of multi-seams in microtubules assem

SUBMITTER: Bousquet C 

PROVIDER: S-EPMC10366996 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature