Whole-body bioluminescence imaging of T-cell response in PDAC models.

ABSTRACT:

Introduction

The location of T-cells during tumor progression and treatment provides crucial information in predicting the response in vivo.

Methods

Here, we investigated, using our bioluminescent, dual color, T-cell reporter mouse, termed TbiLuc, T-cell location and function during murine PDAC tumor growth and checkpoint blockade treatment with anti-PD-1 and anti-CTLA-4. Using this model, we could visualize T-cell location and function in the tumor and the surrounding tumor microenvironment longitudinally. We used murine PDAC clones that formed in vivo tumors with either high T-cell infiltration (immunologically 'hot') or low T-cell infiltration (immunologically 'cold').

Results

Differences in total T-cell bioluminescence could be seen between the 'hot'

SUBMITTER: McMorrow R 

PROVIDER: S-EPMC10367003 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature