Development of Highly Fluorogenic Styrene Probes for Visualizing RNA in Live Cells.

ABSTRACT: Styrene dyes are useful imaging probes and fluorescent sensors due to their strong fluorogenic responses to environmental changes or binding macromolecules. Previously, indole-containing styrene dyes have been reported to selectively bind RNA in the nucleolus and cytoplasm. However, the application of these indole-based dyes in cell imaging is limited by their moderate fluorescence enhancement and quantum yields, as well as relatively high background associated with these green-emitting dyes. In this work, we have investigated the positional and electronic effects of the electron donor by generating regioisomeric and isosteric analogues of the indole ring. Select probes exhibited large Stokes shifts, enhanced molar extinction coefficients, and bathochromic shifts in their absorption and fl

SUBMITTER: Kim MJ 

PROVIDER: S-EPMC10367048 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature