Cellular Exposure to Chloroacetanilide Herbicides Induces Distinct Protein Destabilization Profiles.

ABSTRACT: Herbicides in the widely used chloroacetanilide class harbor a potent electrophilic moiety, which can damage proteins through nucleophilic substitution. In general, damaged proteins are subject to misfolding. Accumulation of misfolded proteins compromises cellular integrity by disrupting cellular proteostasis networks, which can further destabilize the cellular proteome. While direct conjugation targets can be discovered through affinity-based protein profiling, there are few approaches to probe how cellular exposure to toxicants impacts the stability of the proteome. We apply a quantitative proteomics methodology to identify chloroacetanilide-destabilized proteins in HEK293T cells based on their binding to the H31Q mutant of the human Hsp40 chaperone DNAJB8. We find that a brief cellular

SUBMITTER: Quanrud GM 

PROVIDER: S-EPMC10367052 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature