Systematic Part Transfer by Extending a Modular Toolkit to Diverse Bacteria.

ABSTRACT: It is impractical to develop a new parts collection for every potential host organism. It is well-established that gene expression parts, like genes, are qualitatively transferable, but there is little quantitative information defining transferability. Here, we systematically quantified the behavior of a parts set across multiple hosts. To do this, we developed a broad host range (BHR) plasmid system compatible with the large, modular CIDAR parts collection for E. coli, which we named openCIDAR. This enabled testing of a library of DNA constructs across the Pseudomonadota─Escherichia coli, Pseudomonas putida, Cupriavidus necator, and Komagataeibacter nataicola. Part performance was evaluated with a standardized characterization procedure that quantified e

SUBMITTER: Keating KW 

PROVIDER: S-EPMC10367128 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature