Ontology highlight
ABSTRACT:
SUBMITTER: Liu X
PROVIDER: S-EPMC10367133 | biostudies-literature | 2023 Jul
REPOSITORIES: biostudies-literature
Liu Xiuxia X Sun Manman M Gao Alex Xiong AX Ledesma-Amaro Rodrigo R Fang Qiuwu Q Yang Yankun Y Bai Zhonghu Z
ACS synthetic biology 20230623 7
In synthetic biology, the precise control of gene expression is challenging due to the limited orthogonality of expression elements. Here, to address this issue and improve the reusability of genetic elements, we developed a bicistronic expression cassette in <i>Corynebacterium glutamicum</i> based on a leaderless promoter lacking a 5'UTR. The created leaderless bicistronic design (BCD) significantly improved the orthogonality of expression elements across different genes of interest. We also ex ...[more]