CRISPR-Associated Transposase for Targeted Mutagenesis in Diverse Proteobacteria.

ABSTRACT: Genome editing tools, through the disruption of an organism's native genetic material or the introduction of non-native DNA, facilitate functional investigations to link genotypes to phenotypes. Transposons have been instrumental genetic tools in microbiology, enabling genome-wide, randomized disruption of genes and insertions of new genetic elements. Due to this randomness, identifying and isolating particular transposon mutants (i.e., those with modifications at a genetic locus of interest) can be laborious, often requiring one to sift through hundreds or thousands of mutants. Programmable, site-specific targeting of transposons became possible with recently described CRISPR-associated transposase (CASTs) systems, allowing the streamlined recovery of desired mutants in a single step. Lik

SUBMITTER: Trujillo Rodriguez L 

PROVIDER: S-EPMC10367135 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature