The effect of matrices on the gene expression profile of patient-derived head and neck carcinoma cells for in vitro therapy testing.

ABSTRACT:

Objective

Head and neck squamous cell carcinoma (HNSCC) is a highly aggressive tumor with a 5-year mortality rate of ~ 50%. New in vitro methods are needed for testing patients' cancer cell response to anti-cancer treatments. We aimed to investigate how the gene expression of fresh carcinoma tissue samples and freshly digested single cancer cells change after short-term cell culturing on plastic, Matrigel or Myogel. Additionally, we studied the effect of these changes on the cancer cells' response to anti-cancer treatments.

Materials/methods

Fresh tissue samples from HNSCC patients were obtained perioperatively and single cells were enzymatically isolated and cultured on either plastic, Matrigel or Myogel. We treated the cultured cells with cisplatin, cetuximab, and irradiat

SUBMITTER: Hyytiainen A 

PROVIDER: S-EPMC10367262 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature