The genomic characterization of carbapenem-resistant Serratia marcescens at a tertiary hospital in South Africa.

ABSTRACT:

Background

Serratia marcescens is an opportunistic nosocomial pathogen, and recent reports have highlighted the rapid increase in multidrug resistance in this organism. There is a paucity in genomic data for carbapenem-resistant S. marcescens (CRSM).

Methods

A retrospective cohort study describing laboratory-confirmed CRSM from a tertiary academic hospital in Cape Town, South Africa, for the period 2015-20, was performed. Stored CRSM and contemporary isolates were submitted for WGS using Illumina MiSeq, with the Nextera DNA Flex Library Preparation Kit. Sequence data were analysed in-house using srst2 and Tychus, and CRSM and contemporary isolates were compared.

Results

Twenty-one CRSM and four contemporary isolates were sequenced and analysed. Twenty-four diff

SUBMITTER: Overmeyer AJ 

PROVIDER: S-EPMC10368080 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature