Microenvironmental regulation of T-cells in pulmonary hypertension.

ABSTRACT:

Introduction

In pulmonary hypertension (PH), pulmonary arterial remodeling is often accompanied by perivascular inflammation. The inflammation is characterized by the accumulation of activated macrophages and lymphocytes within the adventitial stroma, which is comprised primarily of fibroblasts. The well-known ability of fibroblasts to secrete interleukins and chemokines has previously been implicated as contributing to this tissue-specific inflammation in PH vessels. We were interested if pulmonary fibroblasts from PH arteries contribute to microenvironmental changes that could activate and polarize T-cells in PH.

Methods

We used single-cell RNA sequencing of intact bovine distal pulmonary arteries (dPAs) from PH and control animals and flow cytometry, mRNA expression analy

SUBMITTER: Plecita-Hlavata L 

PROVIDER: S-EPMC10368362 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature