Hepatocytes reprogram liver macrophages involving control of TGF-β activation, influencing liver regeneration and injury.

ABSTRACT:

Background

Macrophages play an important role in maintaining liver homeostasis and regeneration. However, it is not clear to what extent the different macrophage populations of the liver differ in terms of their activation state and which other liver cell populations may play a role in regulating the same.

Methods

Reverse transcription PCR, flow cytometry, transcriptome, proteome, secretome, single cell analysis, and immunohistochemical methods were used to study changes in gene expression as well as the activation state of macrophages in vitro and in vivo under homeostatic conditions and after partial hepatectomy.

Results

We show that F4/80+/CD11bhi/CD14hi macrophages of the liver are recruited in a C-C motif chemokine receptor (CCR2)-dependent manner and exhibit an

SUBMITTER: Wolf SD 

PROVIDER: S-EPMC10368377 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature