High-plex immunofluorescence imaging and traditional histology of the same tissue section for discovering image-based biomarkers.

ABSTRACT: Precision medicine is critically dependent on better methods for diagnosing and staging disease and predicting drug response. Histopathology using hematoxylin and eosin (H&E)-stained tissue (not genomics) remains the primary diagnostic method in cancer. Recently developed highly multiplexed tissue imaging methods promise to enhance research studies and clinical practice with precise, spatially resolved single-cell data. Here, we describe the 'Orion' platform for collecting H&E and high-plex immunofluorescence images from the same cells in a whole-slide format suitable for diagnosis. Using a retrospective cohort of 74 colorectal cancer resections, we show that immunofluorescence and H&E images provide human experts and machine learning algorithms with complementary information that can be u

SUBMITTER: Lin JR 

PROVIDER: S-EPMC10368530 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature