Antibacterial activity mechanism of coptisine against Pasteurella multocida.

ABSTRACT:

Objective

Pasteurella multocida is a widespread zoonotic pathogen that causes severe damage to the poultry industry. This study focused on the antibacterial effects and mechanism of action of coptisine against P. multocida.

Methods

The minimum inhibitory concentration and half maximal inhibitory concentration of coptisine against P. multocida was measured. Additionally, the effect of coptisine on growth, cell wall, activity of respiratory enzymes, soluble protein content and DNA synthesis were also analyzed. Finally, the effect of coptisine on gene transcription was determined using RNA sequencing.

Results

We demonstrated that coptisine has a strong antibacterial effect against P. multocida, with a minimum inhibitory concentration of 0.125

SUBMITTER: Zhang R 

PROVIDER: S-EPMC10369072 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature