Population Control of Upconversion Energy Transfer for Stimulation Emission Depletion Nanoscopy.

ABSTRACT: Upconverting stimulated emission depletion microscopy (U-STED) is emerging as an effective approach for super-resolution imaging due to its significantly low depletion power and its ability to surpass the limitations of the square-root law and achieve higher resolution. Though the compelling performance, a trade-off between the spatial resolution and imaging quality in U-STED has been recognized in restricting the usability due to the low excitation power drove high depletion efficiency. Moreover, it is a burden to search for the right power relying on trial and error as the underpinning mechanism is unknown. Here, a method is proposed that can easily predict the ideal excitation power for high depletion efficiency with the assistance of the non-saturate excitation based on the dynamic cro

SUBMITTER: Liu Y 

PROVIDER: S-EPMC10369235 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature