Non-viral gene delivery to human mesenchymal stem cells: a practical guide towards cell engineering.

ABSTRACT: In recent decades, human mesenchymal stem cells (hMSCs) have gained momentum in the field of cell therapy for treating cartilage and bone injuries. Despite the tri-lineage multipotency, proliferative properties, and potent immunomodulatory effects of hMSCs, their clinical potential is hindered by donor variations, limiting their use in medical settings. To address this challenge, gene delivery technologies have emerged as a promising approach to modulate the phenotype and commitment of hMSCs towards specific cell lineages, thereby enhancing osteochondral repair strategies. This review provides a comprehensive overview of current non-viral gene delivery approaches used to engineer MSCs, highlighting key factors such as the choice of nucleic acid or delivery vector, transfection strategies,

SUBMITTER: Carballo-Pedrares N 

PROVIDER: S-EPMC10369726 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature