Distinct phases of cellular signaling revealed by time-resolved protein synthesis.

ABSTRACT: The post-translational regulation of protein function is involved in most cellular processes. As such, synthetic biology tools that operate at this level provide opportunities for manipulating cellular states. Here, we deploy a proximity-triggered protein trans-splicing technology to enable the time-resolved synthesis of target proteins from pre-made parts. The modularity of the strategy allows for the addition or removal of various control elements as a function of the splicing reaction, in the process permitting the cellular location and/or activity state of starting materials and products to be differentiated. The approach is applied to a diverse set of proteins, including the kinase oncofusions BCR/ABL and DNAJB1/PRKACA where dynamic cellular phosphorylation events are dissected, revea

SUBMITTER: Lee G 

PROVIDER: S-EPMC10369872 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature