DNA satellite and chromatin organization at house mouse centromeres and pericentromeres.

ABSTRACT: Centromeres are essential for faithful chromosome segregation during mitosis and meiosis. However, the organization of satellite DNA and chromatin at mouse centromeres and pericentromeres is poorly understood due to the challenges of sequencing and assembling repetitive genomic regions. Using recently available PacBio long-read sequencing data from the C57BL/6 strain and chromatin profiling, we found that contrary to the previous reports of their highly homogeneous nature, centromeric and pericentromeric satellites display varied sequences and organization. We find that both centromeric minor satellites and pericentromeric major satellites exhibited sequence variations within and between arrays. While most arrays are continuous, a significant fraction is interspersed with non-satellite seq

SUBMITTER: Packiaraj J 

PROVIDER: S-EPMC10370071 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature