Cold temperature induces a TRPM8-independent calcium release from the endoplasmic reticulum in human platelets.

ABSTRACT: Platelets are sensitive to temperature changes and akin to sensory neurons, are activated by a decrease in temperature. However, the molecular mechanism of this temperature-sensing ability is unknown. Yet, platelet activation by temperature could contribute to numerous clinical sequelae, most importantly to reduced quality of ex vivo-stored platelets for transfusion. In this interdisciplinary study, we present evidence for the expression of the temperature-sensitive ion channel transient receptor potential cation channel subfamily member 8 (TRPM8) in human platelets and precursor cells. We found the TRPM8 mRNA and protein in MEG-01 cells and platelets. Inhibition of TRPM8 prevented temperature-induced platelet activation and shape change. However, chemical agonists of TRPM8 did not

SUBMITTER: Stratiievska A 

PROVIDER: S-EPMC10370076 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature