A paradigm for ethanol consumption in head-fixed mice during prefrontal cortical two-photon calcium imaging.

ABSTRACT: The prefrontal cortex (PFC) is a hub for higher-level cognitive behaviors and is a key target for neuroadaptations in alcohol use disorders. Preclinical models of ethanol consumption are instrumental for understanding how acute and repeated drinking affects PFC structure and function. Recent advances in genetically encoded sensors of neuronal activity and neuromodulator release combined with functional microscopy (multiphoton and one-photon widefield imaging) allow multimodal in-vivo PFC recordings at subcellular and cellular scales. While these methods could enable a deeper understanding of the relationship between alcohol and PFC function/dysfunction, they require animals to be head-fixed. Here, we present a method in mice for binge-like ethanol consumption during head-fixation. M

SUBMITTER: Kalelkar A 

PROVIDER: S-EPMC10370124 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature