Role of distal arginine residue in the mechanism of heme nitrite reductases.

ABSTRACT: Heme nitrite reductases reduce NO₂^- by 1e^-/2H⁺ to NO or by 6e^-/8H⁺ to NH₄⁺ which are key steps in the global nitrogen cycle. Second-sphere residues, such as arginine (with a guanidine head group), are proposed to play a key role in the reaction by assisting substrate binding and hydrogen bonding and by providing protons to the active site for the reaction. The reactivity of an iron porphyrin with a NO₂^- covalently attached to a guanidinium arm in its 2nd sphere was investigated to understand the role of arginine residues in the 2nd sphere of heme nitrite reductases. The presence of the guanidinium residue allows the synthetic ferrous porphyrin to reduce NO₂^- a

SUBMITTER: Sarkar A 

PROVIDER: S-EPMC10370594 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature