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Characterization of the Bradyrhizobium japonicum ftsH gene and its product.

ABSTRACT: The Bradyrhizobium japonicum ftsH gene was cloned by using a set of widely applicable degenerated oligonucleotides. Western blot experiments indicated that the FtsH protein was produced under standard growth conditions and that it was not heat inducible. Attempts to delete the ftsH gene in B. japonicum failed, suggesting a pivotal cellular function of this gene. The expression of B. japonicum ftsH in an ftsH-negative Escherichia coli strain significantly enhanced the fitness of this mutant and reduced the steady-state level of sigma(32).

SUBMITTER: Narberhaus F 

PROVIDER: S-EPMC103706 | biostudies-literature | 1999 Dec

REPOSITORIES: biostudies-literature

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Characterization of the Bradyrhizobium japonicum ftsH gene and its product.

Narberhaus F F   Urech C C   Hennecke H H  

Journal of bacteriology 19991201 23

The Bradyrhizobium japonicum ftsH gene was cloned by using a set of widely applicable degenerated oligonucleotides. Western blot experiments indicated that the FtsH protein was produced under standard growth conditions and that it was not heat inducible. Attempts to delete the ftsH gene in B. japonicum failed, suggesting a pivotal cellular function of this gene. The expression of B. japonicum ftsH in an ftsH-negative Escherichia coli strain significantly enhanced the fitness of this mutant and r  ...[more]

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