RNA covariation at helix-level resolution for the identification of evolutionarily conserved RNA structure.

ABSTRACT: Many biologically important RNAs fold into specific 3D structures conserved through evolution. Knowing when an RNA sequence includes a conserved RNA structure that could lead to new biology is not trivial and depends on clues left behind by conservation in the form of covariation and variation. For that purpose, the R-scape statistical test was created to identify from alignments of RNA sequences, the base pairs that significantly covary above phylogenetic expectation. R-scape treats base pairs as independent units. However, RNA base pairs do not occur in isolation. The Watson-Crick (WC) base pairs stack together forming helices that constitute the scaffold that facilitates the formation of the non-WC base pairs, and ultimately the complete 3D structure. The helix-forming WC base pairs car

SUBMITTER: Rivas E 

PROVIDER: S-EPMC10370758 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature