High-altitude cerebral hypoxia promotes mitochondrial dysfunction and apoptosis of mouse neurons.

ABSTRACT:

Introduction

Neuronal cell death is an important factor in the pathogenesis of acute high-altitude cerebral hypoxia; however, the underlying molecular mechanism remains unclear. In this study, we tested if high-altitude hypoxia (HAH) causes neuronal death and mitochondrial dysfunction using various in vivo and in vitro approaches.

Methods

Acute high-altitude cerebral hypoxia was induced by hypobaric hypoxia chamber in male mice. we explored the mechanisms of neuronal cell death using immunofluorescence, western blotting, transmission electron microscopy, and flow cytometry. Next, mitochondrial function and morphology were observed using Jc-1 staining, seahorse assay, western blotting, MitoTracker staining, and transmission electron microscopy. Moreover, open fi

SUBMITTER: Huan Y 

PROVIDER: S-EPMC10370763 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature