Dataset Information

Increase of Severe Pulmonary Infections in Adults Caused by M1_UK Streptococcus pyogenes, Central Scotland, UK.

ABSTRACT: We characterized the epidemiology, host-pathogen characteristics, and outcomes of severe adult pulmonary Streptococcus pyogenes infections that coincided with a high community caseload in central Scotland, UK. The pulmonary infections had high illness and death rates and were associated with socioeconomic deprivation, influenza A co-infection, and the M1_UK lineage of S. pyogenes.

SUBMITTER: Davies PJB

PROVIDER: S-EPMC10370863 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature

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Increase of Severe Pulmonary Infections in Adults Caused by M1<sub>UK</sub> Streptococcus pyogenes, Central Scotland, UK.

Davies Peter J B PJB Russell Clark D CD Morgan Anna-Rose AR Taori Surabhi K SK Lindsay Diane D Ure Roisin R Brown Derek D Smith Andrew A

Emerging infectious diseases 20230621 8

We characterized the epidemiology, host-pathogen characteristics, and outcomes of severe adult pulmonary Streptococcus pyogenes infections that coincided with a high community caseload in central Scotland, UK. The pulmonary infections had high illness and death rates and were associated with socioeconomic deprivation, influenza A co-infection, and the M1<sub>UK</sub> lineage of S. pyogenes. ...[more]

PMID: 37343545

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Project description:Streptococcus pyogenes genotype emm1 is a successful, globally distributed epidemic clone that is regarded as inherently virulent. An emm1 sublineage, M1UK, that produces increased levels of SpeA toxin was associated with increased scarlet fever and invasive infections in England in 2015/2016. Defined by 27 SNPs in the core genome, M1UK is now dominant in England. To more fully characterize M1UK, we undertook comparative transcriptomic and proteomic analyses of M1UK and contemporary non-M1UK emm1 strains (M1global). Just seven genes were differentially expressed by M1UK compared with contemporary M1global strains. In addition to speA, five genes in the operon that includes glycerol dehydrogenase were upregulated in M1UK (gldA, mipB/talC, pflD, and phosphotransferase system IIC and IIB components), while aquaporin (glpF2) was downregulated. M1UK strains have a stop codon in gldA. Deletion of gldA in M1global abrogated glycerol dehydrogenase activity, and recapitulated upregulation of gene expression within the operon that includes gldA, consistent with a feedback effect. Phylogenetic analysis identified two intermediate emm1 sublineages in England comprising 13/27 (M113SNPs) and 23/27 SNPs (M123SNPs), respectively, that had failed to expand in the population. Proteomic analysis of invasive strains from the four phylogenetic emm1 groups highlighted sublineage-specific changes in carbohydrate metabolism, protein synthesis and protein processing; upregulation of SpeA was not observed in chemically defined medium. In rich broth, however, expression of SpeA was upregulated ~10-fold in both M123SNPs and M1UK sublineages, compared with M113SNPs and M1global. We conclude that stepwise accumulation of SNPs led to the emergence of M1UK. While increased expression of SpeA is a key indicator of M1UK and undoubtedly important, M1UK strains have outcompeted M123SNPs and other emm types that produce similar or more superantigen toxin. We speculate that an accumulation of adaptive SNPs has contributed to a wider fitness advantage in M1UK on an inherently successful emm1 streptococcal background.

Dataset Information

Increase of Severe Pulmonary Infections in Adults Caused by M1_UK Streptococcus pyogenes, Central Scotland, UK.

Publications

Increase of Severe Pulmonary Infections in Adults Caused by M1<sub>UK</sub> Streptococcus pyogenes, Central Scotland, UK.

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Dataset Information

Increase of Severe Pulmonary Infections in Adults Caused by M1UK Streptococcus pyogenes, Central Scotland, UK.

Publications

Increase of Severe Pulmonary Infections in Adults Caused by M1<sub>UK</sub> Streptococcus pyogenes, Central Scotland, UK.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Increase of Severe Pulmonary Infections in Adults Caused by M1_UK Streptococcus pyogenes, Central Scotland, UK.