Reverse engineering DNA origami nanostructure designs from raw scaffold and staple sequence lists.

ABSTRACT: Designs for scaffolded DNA origami nanostructures are commonly and minimally published as the list of DNA staple and scaffold sequences required. In nearly all cases, high-level editable design files (e.g. caDNAno) which generated the low-level sequences are not made available. This de facto 'raw sequence' exchange format allows published origami designs to be re-attempted in the laboratory by other groups, but effectively stops designs from being significantly modified or re-purposed for new future applications. To make the raw sequence exchange format more accessible to further design and engineering, in this work we propose the first algorithmic solution to the inverse problem of converting staple/scaffold sequences back to a 'guide schematic' resembling the original origami schematic.

SUBMITTER: Shirt-Ediss B 

PROVIDER: S-EPMC10371787 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature