Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches.

ABSTRACT: Secondary human lymphoid tissue immune reactions take place in a highly coordinated environment with compartmentalization representing a fundamental feature of this organization. In situ profiling methodologies are indispensable for the understanding of this compartmentalization. Here, we propose a complementary experimental approach aiming to reveal different aspects of this process. The analysis of human tonsils, using a combination of single cell phenotypic analysis based on flow cytometry and multiplex imaging and mass spectrometry-based methodologies, revealed a compartmentalized organization at the cellular and molecular levels. More specifically, the skewed distribution of highly specialized immune cell subsets and relevant soluble mediators was accompanied by a compartmental

SUBMITTER: Oyler BL 

PROVIDER: S-EPMC10371825 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature