High throughput human genotyping for variants associated with malarial disease outcomes using custom targeted amplicon sequencing.

ABSTRACT: Malaria has exhibited the strongest known selective pressure on the human genome in recent history and is the evolutionary driving force behind genetic conditions, such as sickle-cell disease, glucose-6-phosphatase deficiency, and some other erythrocyte defects. Genomic studies (e.g., The 1000 Genomes project) have provided an invaluable baseline for human genetics, but with an estimated two thousand ethno-linguistic groups thought to exist across the African continent, our understanding of the genetic differences between indigenous populations and their implications on disease is still limited. Low-cost sequencing-based approaches make it possible to target specific molecular markers and genes of interest, leading to potential insights into genetic diversity. Here we demonstrate the versa

SUBMITTER: Osborne A 

PROVIDER: S-EPMC10371994 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature