Generation of rat offspring using spermatids produced through in vitro spermatogenesis.

ABSTRACT: An in vitro spermatogenesis method using mouse testicular tissue to produce fertile sperm was established more than a decade ago. Although this culture method has generally not been effective in other animal species, we recently succeeded in improving the culture condition to induce spermatogenesis of rats up to the round spermatid stage. In the present study, we introduced acrosin-EGFP transgenic rats in order to clearly monitor the production of haploid cells during spermatogenesis in vitro. In addition, a metabolomic analysis of the culture media during cultivation revealed the metabolic dynamics of the testis tissue. By modifying the culture media based on these results, we were able to induce rat spermatogenesis repeatedly up to haploid cell production, including the formation of elon

SUBMITTER: Matsumura T 

PROVIDER: S-EPMC10372019 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature