Development of rat duodenal monolayer model with effective barrier function from rat organoids for ADME assay.

ABSTRACT: The in-depth analysis of the ADME profiles of drug candidates using in vitro models is essential for drug development since a drug's exposure in humans depends on its ADME properties. In contrast to efforts in developing human in vitro absorption models, only a limited number of studies have explored models using rats, the most frequently used species in in vivo DMPK studies. In this study, we developed a monolayer model with an effective barrier function for ADME assays using rat duodenal organoids as a cell source. At first, we developed rat duodenal organoids according to a previous report, but they were not able to generate a confluent monolayer. Therefore, we modified organoid culture protocols and developed cyst-enriched organoids; these strongly promoted the formation of a confluent

SUBMITTER: Tanaka K 

PROVIDER: S-EPMC10372144 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature