ALS plasma reduces the viability of NSC34 cells via altering mRNA expression of VEGF: A short report.

ABSTRACT:

Introduction

Amyotrophic Lateral Sclerosis (ALS) is a devastating neurodegenerative disorder that progressively leads to motor neuron degeneration at the neuromuscular junctions, resulting in paralysis in the patients. The clinical diagnosis of ALS is time taking and further delays the therapeutics that can be helpful if the disease is diagnosed at an early stage. Changes in plasma composition can be reflected upon CSF composition and hence, can be used to study the diagnosis and prognosis markers for the disease.

Aim

To develop a simple model system using motor neuron like cell line after plasma induction.

Method

Neuroblastoma × Spinal Cord hybridoma cell line (NSC34) was cultured under appropriate conditions. 10% ALS patients' plasma was added to the media, and cell

SUBMITTER: Khosla R 

PROVIDER: S-EPMC10372388 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature