Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni.

ABSTRACT: In living cells, the genetic information stored in the DNA sequence is always associated with chromosomal and extra-chromosomal epigenetic information. Chromatin is formed by the DNA and associated proteins, in particular histones. Covalent histone modifications are important bearers of epigenetic information and as such have been increasingly studied since about the year 2000. One of the principal techniques to gather information about the association between DNA and modified histones is chromatin immunoprecipitation (ChIP), also combined with massive sequencing (ChIP-Seq). Automated ChIPmentation procedure is a convenient alternative to native chromatin immunoprecipitation (N-ChIP). It is now routinely used for ChIP-Seq in many model species, using in general roughly 10 ⁶ cell

SUBMITTER: Lasica C 

PROVIDER: S-EPMC10372461 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature