Adipocyte hypertrophy in mesenchymal stem cells from infants of mothers with obesity.

ABSTRACT:

Objective

Fat content of adipocytes derived from infant umbilical cord mesenchymal stem cells (MSCs) predicts adiposity in children through 4 to 6 years of age. This study tested the hypothesis that MSCs from infants born to mothers with obesity (Ob-MSCs) exhibit adipocyte hypertrophy and perturbations in genes regulating adipogenesis compared with MSCs from infants of mothers with normal weight (NW-MSCs).

Methods

Adipogenesis was induced in MSCs embedded in three-dimensional hydrogel structures, and cell size and number were measured by three-dimensional imaging. Proliferation and protein markers of proliferation and adipogenesis in undifferentiated and adipocyte differentiating cells were measured. RNA sequencing was performed to determine pathways linked to adipogenesis p

SUBMITTER: Keleher MR 

PROVIDER: S-EPMC10372711 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature