Chromatin architectural factor CTCF is essential for progesterone-dependent uterine maturation.

ABSTRACT: Receptors for estrogen and progesterone frequently interact, via Cohesin/CTCF loop extrusion, at enhancers distal from regulated genes. Loss-of-function CTCF mutation in >20% of human endometrial tumors indicates its importance in uterine homeostasis. To better understand how CTCF-mediated enhancer-gene interactions impact endometrial development and function, the Ctcf gene was selectively deleted in female reproductive tissues of mice. Prepubertal Ctcf^d/d uterine tissue exhibited a marked reduction in the number of uterine glands compared to those without Ctcf deletion (Ctcf^f/f mice). Post-pubertal Ctcf^d/d uteri were hypoplastic with significant reduction in both the amount of the endometrial stroma and number of glands. Transcriptional profiling revealed

SUBMITTER: Hewitt SC 

PROVIDER: S-EPMC10372848 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature