In vitro differentiated human CD4⁺ T cells produce hepatocyte growth factor.

ABSTRACT: Differentiation of naive CD4⁺ T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4⁺ T cells can produce hepatocyte growth factor (HGF), a pleiotropic cytokine which can affect several tissue types through signaling by its receptor, c-Met. In vitro differentiation of T cells into Th-like subsets revealed that HGF producing T cells increase under Th1 conditions. Enrichment of HGF producing cells was possible by targeting cells with surface CD30 expression, a marker discovered through single-cell RNA-sequencing. Furthermore, pharmacological inhibition of PI3K or mTOR was found t

SUBMITTER: Ford SL 

PROVIDER: S-EPMC10374024 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature