Structural and biochemical characterization of the key components of an auxin degradation operon from the rhizosphere bacterium Variovorax.

ABSTRACT: Plant-associated bacteria play important regulatory roles in modulating plant hormone auxin levels, affecting the growth and yields of crops. A conserved auxin degradation (iad) operon was recently identified in the Variovorax genomes, which is responsible for root growth inhibition (RGI) reversion, promoting rhizosphere colonization and root growth. However, the molecular mechanism underlying auxin degradation by Variovorax remains unclear. Here, we systematically screened Variovorax iad operon products and identified 2 proteins, IadK2 and IadD, that directly associate with auxin indole-3-acetic acid (IAA). Further biochemical and structural studies revealed that IadK2 is a highly IAA-specific ATP-binding cassette (ABC) transporter solute-binding protein (SBP), likely involved in IAA upta

SUBMITTER: Ma Y 

PROVIDER: S-EPMC10374108 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature