Evaluation of an inducible knockout system in insect cells based on co-infection and CRISPR/Cas9.

ABSTRACT: Due to comparably high product titers and low production costs, the baculovirus/insect cell expression system is considered a versatile production platform in the biopharmaceutical industry. Its excellence in producing complex multimeric protein assemblies, including virus-like particles (VLPs), which are considered promising vaccine candidates to counter emerging viral threats, made the system even more attractive. However, the co-formation of budded baculovirus during VLP production poses a severe challenge to downstream processing. In order to reduce the amount of budded baculovirus in the expression supernatant we developed an inducible knockout system based on CRISPR/Cas9 and co-infection with two baculoviral vectors: one bringing along the Cas9 nuclease and the other one having incor

SUBMITTER: Hausjell CS 

PROVIDER: S-EPMC10374150 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature