GPR81-mediated reprogramming of glucose metabolism contributes to the immune landscape in breast cancer.

ABSTRACT:

Background

Local tumor microenvironment (TME) plays a crucial role in immunotherapy for breast cancer (BC). Whereas, the molecular mechanism responsible for the crosstalk between BC cells and surrounding immune cells remains unclear. The present study aimed to determine the interplay between GPR81-mediated glucometabolic reprogramming of BC and the immune landscape in TME.

Materials and methods

Immunohistochemistry (IHC) assay was first performed to evaluate the association between GPR81 and the immune landscape. Then, several stable BC cell lines with down-regulated GPR81 expression were established to directly identify the role of GPR81 in glucometabolic reprogramming, and western blotting assay was used to detect the underlying molecular mechanism. Finally, a transwell co

SUBMITTER: Li X 

PROVIDER: S-EPMC10374510 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature