Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications.

ABSTRACT: Probiotics are defined as live organisms in the host that contribute to health benefits. Lactobacillus gasseri LM1065, isolated from human breast milk, was investigated for its probiotic properties based on its genome. Draft genome map and de novo assembly were performed using the PacBio RS II system and hierarchical genome assembly process (HGAP). Probiotic properties were determined by the resistance to gastric conditions, adherence ability, enzyme production, safety assessment and mobile genetic elements. The fungistatic effect and inhibition of hyphae transition were studied using the cell-free supernatant (CFS). L. gasseri LM1065 showed high gastric pepsin tolerance and mild tolerance to bile salts. Auto-aggregation and hydrophobicity were measured to be 61.21% and 61.55%, respectivel

SUBMITTER: Bae WY 

PROVIDER: S-EPMC10374649 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature