Integrative analyses of bulk microarray data to discover genes, pathways, and immune infiltration characteristics associated with targeting of Ewing sarcoma.

ABSTRACT:

Purpose

To explore transcriptome and immunological features of patients with Ewing sarcoma (ES) using all publicly available microarray data.

Methods

Data of 479 ES tissues were integrated and normalized. Gene expression, immune infiltration, and cancer-specific pathways were analyzed. Genes of interest were knocked down, followed by cell proliferation and colony formation assays.

Results

Consistent with the previous reports of differential expressed genes (DEGs) in ES, our analysis identified CCND1, HMCN1, and NKX2-2 were among the most highly expressed, while TWNC1, MYBPC1, and CKM were among the lowest expressed genes. GO, KEGG, and GSEA enrichment analysis identified that the DEGs related to bone and muscle functioning, those that contributed to crucial cellular,

SUBMITTER: Cao B 

PROVIDER: S-EPMC10374716 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature