Expression profiling of N6-methyladenosine-modified mRNA in PC12 cells in response to unconjugated bilirubin.

ABSTRACT:

Background

Abnormal methylation of N⁶-methyladenosine (m⁶A) is reportedly associated with central nervous system disorders. However, the role of m⁶A mRNA methylation in unconjugated bilirubin (UCB) neurotoxicity requires further research.

Methods

Rat pheochromocytoma PC12 cells treated with UCB were used as in vitro models. After the PC12 cells were treated with UCB (0, 12, 18, and 24 µM) for 24 h, the total RNA m⁶A levels were measured using an m⁶A RNA methylation quantification kit. The expression of m6A demethylases and methyltransferases was detected through western blotting. We determined the m⁶A mRNA methylation profile in PC12 cells exposed to UCB (0 and 18 µM) for 24 h using methylated RNA immunoprecip

SUBMITTER: Zhou J 

PROVIDER: S-EPMC10374823 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature