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Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes.

ABSTRACT: Atomic force microscopy (AFM) is capable of nanoscale imaging but has so far only been used on cell surfaces when applied to a living cell. Here, we describe a step-by-step protocol for nanoendoscopy-AFM, which enables the imaging of nanoscale structures inside living cells. The protocol consists of cell staining, fabrication of the nanoneedle probes, observation inside living cells using 2D and 3D nanoendoscopy-AFM, and visualization of the 3D data. For complete details on the use and execution of this protocol, please refer to Penedo et al. (2021)¹ and Penedo et al. (2021).².

SUBMITTER: Ichikawa T

PROVIDER: S-EPMC10374873 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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Protocol for live imaging of intracellular nanoscale structures using atomic force microscopy with nanoneedle probes.

Ichikawa Takehiko T Alam Mohammad Shahidul MS Penedo Marcos M Matsumoto Kyosuke K Fujita Sou S Miyazawa Keisuke K Furusho Hirotoshi H Miyata Kazuki K Nakamura Chikashi C Fukuma Takeshi T

STAR protocols 20230722 3

Atomic force microscopy (AFM) is capable of nanoscale imaging but has so far only been used on cell surfaces when applied to a living cell. Here, we describe a step-by-step protocol for nanoendoscopy-AFM, which enables the imaging of nanoscale structures inside living cells. The protocol consists of cell staining, fabrication of the nanoneedle probes, observation inside living cells using 2D and 3D nanoendoscopy-AFM, and visualization of the 3D data. For complete details on the use and execution ...[more]

PMID: 37481726

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