Activating transcription factor 4 drives the progression of diabetic cardiac fibrosis.

ABSTRACT:

Aims

Diabetic cardiomyopathy (DC) is one of serious complications of diabetic patients. This study investigated the biological function of activating transcription factor 4 (ATF4) in DC.

Methods and results

Streptozotocin-treated mice and high glucose (HG)-exposed HL-1 cells were used as the in vivo and in vitro models of DC. Myocardial infarction (MI) was induced by left coronary artery ligation in mice. Cardiac functional parameters were detected by echocardiography. Target molecule expression was determined by real time quantitative PCR and western blotting. Cardiac fibrosis was observed by haematoxylin and eosin and Masson's staining. Cardiac apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick end labelling. Activities of superoxide dismutase, glut

SUBMITTER: Li Y 

PROVIDER: S-EPMC10375070 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature