FASTdRNA: a workflow for the analysis of ONT direct RNA sequencing.

ABSTRACT:

Motivation

Direct RNA-seq (dRNA-seq) using Oxford Nanopore Technology (ONT) has revolutionized transcript mapping by offering enhanced precision due to its long-read length. Unlike traditional techniques, dRNA-seq eliminates the need for PCR amplification, reducing the impact of GC bias, and preserving valuable base physical information, such as RNA modification and poly(A) length estimation. However, the rapid advancement of ONT devices has set higher standards for analytical software, resulting in potential challenges of software incompatibility and reduced efficiency.

Results

We present a novel workflow, called FASTdRNA, to manipulate dRNA-seq data efficiently. This workflow comprises two modules: a data preprocessing module and a data analysis module. The preprocessing d

SUBMITTER: Chen X 

PROVIDER: S-EPMC10375421 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature