Development of a mammalian cell-based ZZ display system for IgG quantification.

ABSTRACT:

Background

Biological laboratories and companies involved in antibody development need convenient and versatile methods to detect highly active antibodies.

Methods

To develop a mammalian cell-based ZZ display system for antibody quantification, the eukaryotic ZZ-displayed plasmid was constructed and transfected into CHO cells. After screening by flow cytometric sorting, the stable ZZ display cells were incubated with reference IgG and samples with unknown IgG content for 40 min at 4℃, the relative fluorescence intensity of cells was analyzed and the concentration of IgG was calculated.

Results

By investigating the effects of different display-associated genetic elements, a eukaryotic ZZ-displaying plasmid with the highest display efficiency were constructed. After tra

SUBMITTER: Bao L 

PROVIDER: S-EPMC10375748 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature