Heteroresistance to Colistin in Clinical Isolates of Klebsiella pneumoniae Producing OXA-48.

ABSTRACT: Heteroresistance to colistin can be defined as the presence of resistant subpopulations in an isolate that is susceptible to this antibiotic. Colistin resistance in Gram-negative bacteria is more frequently related to chromosomal mutations and insertions. This work aimed to study heteroresistance in nine clinical isolates of Klebsiella pneumoniae producing OXA-48 and to describe genomic changes in mutants with acquired resistance in vitro. Antimicrobial susceptibility was determined by broth microdilution (BMD) and heteroresistance by population analysis profiling (PAP). The proteins related to colistin resistance were analyzed for the presence of mutations. Additionally, PCR of the mgrB gene was performed to identify the presence of insertions. In the nine parental isolates,

SUBMITTER: Sanchez-Leon I 

PROVIDER: S-EPMC10375995 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature