Secretion of Interleukin 6 in Human Skeletal Muscle Cultures Depends on Ca²⁺ Signalling.

ABSTRACT: The systemic effects of physical activity are mediated by the release of IL-6 and other myokines from contracting muscle. Although the release of IL-6 from muscle has been extensively studied, the information on the cellular mechanisms is fragmentary and scarce, especially regarding the role of Ca²⁺ signals. The aim of this study was to characterize the role of the main components of Ca²⁺ signals in human skeletal muscle cells during IL-6 secretion stimulated by the Ca²⁺ mobilizing agonist ATP. Primary cultures were prepared from surgical samples, fluorescence microscopy was used to evaluate the Ca²⁺ signals and the stimulated release of IL-6 into the medium was determined using ELISA. Intracellular calcium chelator Bapta, low extracellular calci

SUBMITTER: Calle-Ciborro B 

PROVIDER: S-EPMC10376320 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature