Genetic Markers for Metabarcoding of Freshwater Microalgae: Review.

ABSTRACT: The metabarcoding methods for studying the diversity of freshwater microalgae and routine biomonitoring are actively used in modern research. A lot of experience has been accumulated already, and many methodological questions have been solved (such as the influence of the methods and time of sample conservation, DNA extraction and bioinformatical processing). The reproducibility of the method has been tested and confirmed. However, one of the main problems-choosing a genetic marker for the study-still lacks a clear answer. We analyzed 70 publications and found out that studies on eukaryotic freshwater microalgae use 12 markers (different nuclear regions 18S and ITS and plastids rbcL, 23S and 16S). Each marker has its peculiarities; they amplify differently and have various levels of

SUBMITTER: Kezlya E 

PROVIDER: S-EPMC10376359 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature